FIG. 2.
Determination of the NS3 and E2 proteins in the stable cell lines by Western blotting. A total of 25 μg of cell extract was analyzed in a 10% sodium dodecyl sulfate-polyacrylamide gel. The NS3 and E2 proteins were detected by Western blotting using monoclonal antibodies against NS3 and E2 proteins (see Materials and Methods). The stable cell lines are numbered on the top. The naïve parental cell extract (Mock) was used as a negative control, and the β-actin protein was used as an internal control to normalize the amounts of proteins.