FIG. 3.
Activation of MAPK in EHEC-infected T84 cells. T84 cells were lysed at different times after infection. Samples were resolved by SDS-PAGE and analyzed by immunoblotting using anti-phospho-ERK1/2 and anti-ERK2 antibodies (A), anti-phospho-p38 and anti-p38 antibodies (B), and anti-phospho-JNK and anti-JNK antibodies (C). The control lane contained uninfected T84 cells. Epidermal growth factor (EGF) (10 nM) was used as the positive control. The increase in MAPK activity was quantified after densitometric analysis.