FIG. 1.
PCR analysis of motB mutants.(A) Schematic representations of wild-type motB (i), motB::cat (ii), and ΔmotB (iii). Arrows represent primers used for PCR. Primer 1 is motB1, primer 2 is motB3, and primer 3 is colicat1 (sequences are in the text). Numbers at the top of panel i indicate nucleotide numbers for the motB open reading frame, while numbers at the bottom represent the genomic sequence numbers from the TIGR sequence (42). The location of the BclI site used for inserting the cat/aphA3-sacB is marked. (B) Agarose gel analysis of PCR amplification products from genomic DNA of SS1, SS1 ΔmotB and SS1 motB::cat, and G27 and G27 motB::cat. The source of genomic DNA is shown at the top of the figure; the primer set (as in panel A) is shown at the bottom. The arrows indicate full-length motB, and the diamonds indicate altered motB (either motB::cat or ΔmotB). Kilobase markers are shown on the left side. Although in some lanes multiple bands can be seen, in all cases the dominant band is the band of interest. Similar results were obtained with G27ΔmotB.