Skip to main content
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1976 Apr;40(2):161–165.

The detection of transmissible gastroenteritis viral antibodies by immunodiffusion.

J Bohac, J B Derbyshire
PMCID: PMC1277542  PMID: 187295

Abstract

Precipitating antibodies against transmissible gastroenteritis viral antigens were detected by the immunodiffusion test in two transmissible gastroenteritis viral hyperimmune antisera and in antiserum prepared against haemagglutinating encephalomyelitis virus but not in sera from several species of normal animals, in antisera prepared against a variety of othet viruses and bacteria or sera from swine with bacterial enteritis. When the immunodiffusion test was compared with the virus neutralization test for the detection of transmissible gastroeneritis viral antibodies in 20 swine sera certain samples which contained high titres of virus neutralizing antibodies failed to produce precipitation while other sera were positive in the immunodiffusion test although their virus neutralizing antibody titres were relatively low. Precipitating antibodies were also detected by immunodiffusion in several samples of milk whey from a sow which had been vaccinated with inactivated transmissible gastroenteritis virus.

Full text

PDF
161

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bohac J., Derbyshire J. B., Thorsen J. The detection of transmissible gastroenteritis viral antigens by immunodiffusion. Can J Comp Med. 1975 Jan;39(1):67–75. [PMC free article] [PubMed] [Google Scholar]
  2. Bourne F. J. IgA immunoglobin from porcine milk. Biochim Biophys Acta. 1969 Jul 1;181(2):485–487. doi: 10.1016/0005-2795(69)90287-6. [DOI] [PubMed] [Google Scholar]
  3. Bradburne A. F. Antigenic relationships amongst coronaviruses. Arch Gesamte Virusforsch. 1970;31(3):352–364. doi: 10.1007/BF01253769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KLONTZ G. W., SVEHAG S. E., GORHAM J. R. A study by the agar diffusion technique of precipitating antibody directed against blue tongue virus and its relation to homotypic neutralizing antibody. Arch Gesamte Virusforsch. 1962;12:259–268. doi: 10.1007/BF01258136. [DOI] [PubMed] [Google Scholar]
  5. Phillip J. I., Cartwright S. F., Scott A. C. The size and morphology of T.G.E. and vomiting and wasting disease viruses of pigs. Vet Rec. 1971 Mar 20;88(12):311–312. doi: 10.1136/vr.88.12.311. [DOI] [PubMed] [Google Scholar]
  6. SCHMIDT N. J., LENNETTE E. H. Gel double diffusion studies with group B and group A, type 9 Coxsackie viruses. I. The technique and reactions obtained with hyperimmune animal sera and human sera. J Immunol. 1962 Jul;89:85–95. [PubMed] [Google Scholar]
  7. Thorsen J., Djurickovic S. Experimental immunization of sows with inactivated transmissible gastroenteritis (TGE) virus. Can J Comp Med. 1971 Apr;35(2):99–102. [PMC free article] [PubMed] [Google Scholar]
  8. Witte K. H. Micro-color test for assay of transmissible gastroenteritis virus-neutralizing antibodies. Arch Gesamte Virusforsch. 1971;33(1):171–176. doi: 10.1007/BF01254174. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES