FIG. 3.
TNF-α is required within the first 48 to 72 h after R36A immunization for induction of an anti-PspA response. Mice were immunized i.p. with R36A (108 CFU/mouse), and then distinct sets of mice were injected i.p. with 0.5 mg of either anti-TNF-α MAb or control MAb at different times (days 0 to 5). Sera were collected 2 weeks after primary immunization with R36A for the determination of anti-PspA titers. Values represent the arithmetic means ± SEM of five mice per group. *, P < 0.05. Data are representative of two similar experiments.