Extended Data Fig. 6: A fat-brain and gut-brain T cell axis exists at homeostasis.
a, Body fat percentage of mice fed a high-fat diet (HFD). (n=3 per group; pooled 2 experiments). b, Tissue CD4 T cell numbers HFD-fed mice. (n=7 per group; pooled 2 experiments). c, Body fat percentage of mice fasted for 48 hours. (n=9 per group; pooled 2 experiments). d, CD4 T cell numbers in mice fasted for 48 hours. (n=3 per group; brain, white-fat, brown-fat pooled 2 experiments; repeated twice). e, Body fat percentage changes in mice fed a HFD and then fasted. (n=3 per group) f, Spleen and bone marrow CD4 T cell numbers as in e. (n=3 per group; spleen=pooled 2 experiments). g, Representative FACS plots from fat-photoconverted mice. h, Brain IFNγ+ and total CD8 T cell numbers at various ages. (n=3 per group; repeated once) i, Total CD4 and CD8 T cells and IFNγ-secreting CD8 T cell numbers in brains of various-aged germ free and SPF mice. (n=3 per group; repeated once). j, FACS analysis of brain CD4 T cells post antibiotic (ABX)-treatment. k, Same as j, except for total CD4 T cells in other tissues. (n=4 per group; repeated once). l Representative FACS plots of adult and m, newly-weaned mice that underwent gut or fat photoconversion. Proportion of photoconverted T cells quantified. (n=3 per group; pooled 2 experiments) n, Brain CD4 T cell numbers in weaning-aged mice treated with anti-VLA4 and anti-LFA1. (n=4 per group) o, Brain CD4 T cell numbers in weaning-aged mice treated with FTY720. (n=4 per group). Statistical tests: Unpaired t-test for a, b, c, d, k, n, o. Two-way ANOVA with Bonferroni’s multiple comparisons test for e, f, i. One-way ANOVA with Tukey’s multiple comparisons test for h, m. For all graphs, *= p<0.05, **=p<0.01, ***=p<0.001, ****= p<0.0001.