Table 1.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| YCplac111 | sc LEU2 cloning vector | Gietz and Sugino (1988) |
| YEplac181 | hc LEU2 cloning vector | Gietz and Sugino (1988) |
| p3392 | sc URA3 TIF11 in YCplac33 | Olsen et al (2003) |
| p3499 | sc LEU2 TIF11-FL in YCplac111 | Olsen et al (2003) |
| p3604 | hc LEU2 tif11-Δ108–153-FL in YEplac181 | Olsen et al (2003) |
| p4399 | sc LEU2 tif11-RKKVW66–70AAAVA-FL in YCplac111 | This study |
| p4443 | hc LEU2 tif11-RKKVW66–70AAAVA-FL in YEplac181 | This study |
| p4398 | sc LEU2 tif11-DEAR98–101AAAA-FL in YCplac111 | This study |
| p4444 | hc LEU2 tif11-DEAR98–101AAAA-FL in YEplac181 | This study |
| p1780-IMT | hc URA3 SUI2, GCD11, SUI3, IMT4 in YEp24 | Asano et al (1999) |
| YEp24 | hc URA3 cloning vector | Parent et al (1985) |
| YEpTIF5-U | hc URA3 TIF5-FL in YEplac195 | Valasek et al (2004) |
| YEplac195 | hc URA3 | Gietz and Sugino (1988) |
| p4385 | hc TRP1 SUI2, GCD11, SUI3, IMT4 in YEp24, ura3∷TRP1:kanR | This study |
| YEplac112 | hc TRP1 | Gietz and Sugino (1988) |
| p180 | sc URA3 GCN4-lacZ with WT leader | Hinnebusch (1985) |
| PM199 | sc URA3 GCN4-lacZ with uORF1 only | Grant et al (1994) |
| PM226 | sc URA3 GCN4-lacZ with uORF1 extending into GCN4 | Grant et al (1994) |
| p367 | sc URA3 AUG-HIS4-lacZ | Donahue and Cigan (1988) |
| p391 | sc URA3 AUU-HIS4-lacZ | Donahue and Cigan (1988) |