Figure 5.
Preferential oxidative mtDNA damage induced by rotenone. (A) HCT116 cells were treated with 300 nM rotenone for 12–24 h as indicated. nDNA and mtDNA were isolated, dot-blotted onto a nitrocellulose membrane, and assayed for 8-oxo-dG residues in DNA using a specific antibody against 8-oxo-dG as described in Materials and methods. (B) Quantitation of oxidative mtDNA damage induced by rotenone. The intensity of the dots of the mtDNA samples was measured by densitometry. The bars show the relative dot intensity compared to the control sample. Data represent mean±s.d. from three separate experiments.