Table 1.
Binding characteristics of different DBLγ to Biot-CSA
| PE phenotype | CIDR1 binding to CD36 | Construct expressed | Binding of Biot-CSA to CHO-745 cells*
|
|||||
|---|---|---|---|---|---|---|---|---|
| No inhibition
|
Inhibition with CSA
|
Inhibition with CSC
|
||||||
| Positive cells,† % | Beads per 100 cells‡ | Positive cells,† % | Beads per 100 cells‡ | Positive cells,† % | Beads per 100 cells‡ | |||
| CSA+/CD36− | − | FCR3-CSA DBL3γ | 93 ± 2 | 1,432 ± 89 | 2 ± 1 | ND | 91 ± 5 | 1,163 ± 24 |
| − | ItG2-CS2 DBL2γ | 76 ± 3 | 1,023 ± 56 | 1 ± 1 | ND | 71 ± 8 | 986 ± 47 | |
| − | FCR3-CSA DBL5γ | 2 ± 1 | ND | ND | ND | ND | ND | |
| CD36+/CSA− | + | A4-tres DBL3γ | 89 ± 3 | 1,356 ± 72 | 0 ± 1 | ND | 92 ± 4 | 1,389 ± 63 |
| + | A4 DBL4γ | 1 ± 1 | ND | ND | ND | ND | ND | |
| Unknown | − | 3D7 AL010226 DBL4γ | 98 ± 1 | 1,548 ± 49 | 3 ± 2 | ND | 97 ± 4 | 1,441 ± 57 |
| ND | FCR3-Var3 DBL3γ | 3 ± 2 | ND | ND | ND | ND | ND | |
ND, not determined.
*
Cells were incubated with Biot-CSA without (control) or after preincubation with 200 μg/ml CSA or CSC.
†
One hundred cells expressing the recombinant protein were checked for the presence of CSA-coated Dynal beads on their surface. Cells with four or more beads attached were considered positive for binding. Results are expressed as the mean and SD of three independent experiments.
‡
The number of beads was counted on 100 cells determined positive for binding to CSA-coated Dynal beads. Results are expressed as the mean and SD of three independent experiments.