. 2005 Oct;25(20):8887–8903. doi: 10.1128/MCB.25.20.8887-8903.2005

TABLE 1.

S. pombe strains used in this study

Strain	Genotype	Source
FY253	h⁺ leu1-32 ade6-M210	Our stock
FY254	h⁻ ura4-D18 leu1-32 ade6	Our stock
FY258	h leu1-32 ade6-M216	Our stock
FY360	h⁺ cdc19-P1 (mcm2ts) ura4-D18 leu1-32 ade6	Our stock
FY421	h⁻ Δchk1::ura4⁺ ura4-D18 leu1-32 ade6	T. Carr
FY784	h⁺ cdc21-M68 (mcm4ts) ura4-D18 leu1-32 ade6	Our stock
FY793	h⁻ orp1HA ura4-D18 leu1-32 ade6	Our stock
FY798	h⁻ Δcdc19::[cdc19HA::leu1⁺] (mcm2HA) ura4-D18 leu1-32 ade6	Our stock
FY865	h⁻ Δcds1::ura4⁺ ura4-D18 leu1-32	D. Griffiths
FY1012	h⁺ Δrec12::LEU2⁺ ura4 leu1-32 ade6	Our stock
FY1027	h⁻ goa1-U53 (sna41ts) ura4 leu1 his3-D1 ade6	Our stock
FY1107	h⁻ Δrad3::ura4⁺ ura4-D18 leu1-32 ade6	Our stock
FY1114	h⁻ rad4-116 ura4-D18 leu1-32 ade6	Our stock
FY1159	h⁻ rad21-K1::ura4⁺ ura4-D18 leu1-32 ade6 his7-366	H. Ikeda
FY1204	h⁺ Δrhp51::ura4⁺ ura4-D18 leu1-32 ade6	A. Lehmann
FY1353	h⁺ pku70::kanMX6 ura4-D18 leu1-32 ade6 his3-D1	P. Bauman
FY1392	h⁻ Δtel1::ura4⁺ ura4-D18 leu1-32 ade6	J. Kanoh
FY1421	h⁻ Δtaz1::ura4⁺ ura4-D18 leu1-32 ade6	M. Ferreira
FY1538	h⁺ eso1-H17 ura4-D18 leu1-32 ade6	Our stock
FY1589	h⁻ imr1L(NcoI)::ura4⁺ ura4-DS/E leu1-32 ade6 his1-102	R. Allshire
FY1592	h⁻ tel1L::ura4⁺ ura4-DS/E leu1-32 ade6 his1-102	R. Allshire
FY1754	h⁺/h⁻ Δmst1::ura4⁺/mst2⁺ ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M216/ade6/M210	This work
FY1812	h⁺ mst1::[mst1-HA leu1⁺]ura4-D18 leu1-32 ade6	This work
FY1818	h⁺ mst2::[mst2-HA leu1⁺] ura4-D18 leu1-32 ade6	This work
FY1886	h⁹⁰ Δchp2::ura4⁺ ura4-D18 leu1-32 ade6	G. Thon
FY1889	h⁻ Δmst2::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY1890	h⁺ Δmst2::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY1918	h⁻ Δmst2::ura4⁺ Δchk1::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2002	h⁻ Δmst2::ura4⁺ goa1-U53 (sna41ts) ura4-D18 leu1-32 ade6 his3-D1	This work
FY2009	h⁻ Δmst2::ura4⁺ eso1-H17 leu1-32 ura4-D18 ade6	This work
FY2019	h⁻ Δchl12::his3⁺ ura4-D18 leu1-32 ade6 his3-D1	Our stock
FY2032	h⁺ Δmst2::ura4⁺ rad21-K1:: ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2046	h⁻ Δmst2::ura4⁺ Δswi6::ura4⁺ ura4 leu1-32 ade6	This work
FY2056	h⁻ Δswi6::ura4⁺ ura4 leu1-32 ade6	This work
FY2081	h⁻ Δmst2::ura4⁺ rad4-116 ura4-D18 leu1-32 ade6	This work
FY2083	h⁺ Δmst2::ura4⁺ cdc19-P1 ura4-D18 leu1-32 ade6	This work
FY2084	h⁻ Δmst2:ura4⁺ cdc21-M68 ura4-D18 leu1-32 ade6	This work
FY2085	h⁺ Δmst2::ura4⁺ hsk1-1312 ura4-D18 leu1-32 ade6	This work
FY2104	h⁻ hsk1-1312 ura4-D18 leu1-32 ade6	Our stock
FY2161	h⁻ Δmst2::ura4⁺ Δchl12::his3⁺ ura4-D18 leu1-32 ade6 his3-D1	This work
FY2213	h⁻ Δhda1::LEU2⁺ leu1-32	K. Ekwall
FY2214	h⁻ clr6-1	K. Ekwall
FY2215	h⁻ Δclr3::kanMX6 ura4-D18 leu1-32 ade6	K. Ekwall
FY2219	h⁺ leu1-32 ura4-D18 ade6-210 otr1R (dg-glu) SphI::ade6⁺	R. Allshire
FY2223	h⁻ (XbaI-SpeI) Δclr4::LEU2⁺ ura4-D18 leu1-32 ade6	R. Allshire
FY2326	h⁻ Δmst2::ura4⁺ Δtel1::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2328	h⁺ leu1-32 ura4ΔS/E ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺	K. Ekwall
FY2330	h⁺ Δmst2::ura4⁺ Δtaz1::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2332	h⁺ Δmst2::ura4⁺ Δrec12::LEU2 ura4-D18 leu1-32 ade6	This work
FY2339	h⁺ Δmst1::ura4⁺ leu1::nmt-mst1L-S ura4-D18 leu1 ade6	This work
FY2392	h⁹⁰ taz1-HA::ura4⁺ura4-D18 leu1-32 ade6	J. Kanoh
FY2349	h⁹⁰ mat3-m(RV)::ade6⁺ ori1 ura4-D18 leu1-32 ade6-DN/N	R. Allshire
FY2364	h⁺ Δmst2::ura4⁺ ch16 M23::LEU2⁺ tel::ade6⁺ ura4 leu1-32 ade6ΔN/N	This work
FY2365	h⁻ Δmst2::ura4⁺ ch16 M23::LEU2⁺ tel::ade6⁺ ura4 leu1-32 ade6ΔN/N	This work
FY2367	h⁺ Δtaz1:ura4⁺ ura4 leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺	This work
FY2412	h⁻ Δmst2::ura4⁺ ura4-D18 leu1-32 ade6ΔN/N otr1R (dg-glu) SphI::ade6⁺	This work
FY2505	h⁺ Δmst2::ura4⁺ mst1::kanMX6 leu1::nmt-mst1L-S-leu1⁺ ura4-D18 leu1-32 ade6	This work
FY2548	h⁻ taz1-GFP::kanMX6 ura4-D18 leu1-32 ade6	This work
FY2550	h⁻ taz1-GFP::kanMX6 Δmst2::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2665	h⁺ Δmst2::ura4⁺ sir2-myc::kanMX6 ura4-D18 leu1-32 ade6	Our stock
FY2670	h⁻ Δmst2::ura4⁺ Δsir2::kanMX6 ura4-D18 leu1-32 ade6	This work
FY2700	h⁺ Δmst2::ura4⁺ Δsir2::kanMX6 ura4 leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺	This work
FY2702	h⁺ Δsir2::kanMX6 ura4 leu1-32 ade6ΔN/N ch16 M23::LEU2+ tel::ade6⁺	This work
FY2752	h⁺ Δmst2::ura4⁺ pku70::kanMX6 ura4-D18 leu1-32 ade6	This work
FY2773	h⁺ sir2-myc::kanMX6 leu1-32 ade6	Our stock
FY2784	h⁻ taz1-HA::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2786	h⁹⁰ taz1-HA::ura4⁺ Δmst2::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2787	h⁻ taz1-HA::ura4⁺ Δmst2::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2897	h⁻ Δmst2::ura4⁺ Δhda1::LEU2⁺ ura4 leu1-32 ade6	This work
FY2902	h⁺ Δmst2::ura4⁺ rad21-3HA-kanMX6 ura4 leu1-32 ade6	This work
FY2903	h⁻ Δmst2::ura4⁺ rad21-3HA-kanMX6 ura4 leu1-32 ade6	This work
FY2938	h⁺ Δmst2::ura4⁺ Δclr4::LEU2⁺ ura4-D18 leu1-32 ade6	This work
FY2940	h⁺ Δmst2::ura4⁺ clr6-1 ura4 ade	This work
FY2948	h⁺ Δmst2::ura4⁺ Δclr3::kanMX6 ura4-D18 leu1-32 ade6	This work
FY2952	h⁺ Δmst2::kanMX6 ura4-D18 leu1-32 ade6	This work
FY2956	h⁹⁰ Δmst2::ura4⁺ Δchp2::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY2996	h⁺ leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺	This work
FY2997	h⁻ leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺	This work
FY3001	h⁹⁰ mat3-m(RV)::ade6⁺ ori1 ade6-DN/N leu1-32	This work
FY3003	h⁹⁰ Δmst2::ura4⁺ mat3-m(RV)::ade6⁺ ori1 ura4-D18 leu1-32 ade6-DN/N	This work
FY3036	h⁻ TM1::ade6⁺ ura4 leu1-32 ade6-DN/N	This work
FY3039	h⁻ TM1::ade6⁺ Δmst2::ura4⁺ ura4 leu1-32 ade6-DN/N	This work
FY3043	h⁻ tel1L::ura4⁺ ura4-DS/E leu1-32 ade6	This work
FY3044	h⁻ Δmst2::kanMX6 tel1L::ura4-⁺ ura4-DS/E leu1-32 ade6	This work
FY3046	h⁺ Δmst2::kanMX6 imr1L(NcoI)::ura4⁺ ura4-DS/E leu1-32 ade6 his1-102	This work
FY3048	h⁺ rad21-3HA-kanMX6 leu1-32 ade6	This work
FY3049	h⁻ rad21-3HA-kanMX6 leu1-32 ade6	This work
FY3169	h⁺ mis4-242 Δmst2::ura4⁺ ura4-D18 leu1-32 ade6	This work
FY3284	h⁻ ade6-210 leu1-32 ura4-DS/E Ch16 ade6-216 M23::TEL::ura4⁺	L. Pillus
FY3289	h⁻ Δmst2::kanMX6 ade6-210 leu1-32 ura4-DS/E Ch16 ade6-216 M23::TEL::ura4⁺ (isolate #1)	This work
FY3290	h⁻ Δmst2::kanMX6 ade6-210 leu1-32 ura4-DS/E Ch16 ade6-216 M23::TEL::ura4⁺ (isolate #2)	This work
FY3291	h⁻ Δmst2::kanMX6 ade6-210 leu1-32 ura4-DS/E Ch16 ade6-216 M23::TEL::ura4⁺ (isolate #3)	This work
FY3292	h⁺ Δmst2::kanMX6 ade6-210 leu1-32 ura4-DS/E Ch16 ade6-216 M23::TEL::ura4⁺ (isolate #4)	This work
FY3293	h⁻ Δswi6::ura4⁺ ura4 leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺	This work
FY2395	h⁻ Δmst2::kanMX6 Δswi6::ura4⁺ ura4 leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺	This work
FY2398	h⁻ Δmst2::kanMX6 Δtaz1::ura4⁺ ura4 leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺ (isolate #5)	This work
FY2399	h⁻ Δmst2::kanMX6 Δtaz1::ura4⁺ ura4 leu1-32 ade6ΔN/N ch16 M23::LEU2⁺ tel::ade6⁺ (isolate #6)	This work