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SDS-polyacrylamide gel electrophoresis of protein purification. Lanes 1 and 5 contain molecular size markers. Lane 2 is clarified cell lysate (13 μg). The rPPK-chitin-binding domain (CBD) fusion protein, with an approximate predicted size of 130 kDa, is identified. Lane 3 is pooled fractions eluted from the chitin beads (0.8 μg). Lane 4 is pooled and concentrated fractions eluted from the size exclusion column (0.8 μg). Proteins were stained with Coomassie blue.
