Fig 1.
(A) Low concentrations of PTX or VCR enhance p53 nuclear accumulation in A549 cells. A549 cells were treated with the indicated drugs for 18 hr and processed for double immunofluorescence labeling with antibodies against p53 (red, labeled with Rhodamine red X) or α-tubulin (green, labeled with FITC). Untreated A549 cells were included as control (CTRL). Staining was analyzed by CLSM. (Bar = 10 μm.) (B) Low concentrations of PTX or VCR induce mdm2 in A549 cells. Untreated A549 cells (0) or cells treated for 18 hr were processed for Western blotting. Cells treated with 400 ng/ml of ADR for 18 hr are included as a positive control for p53 and mdm2 increased levels. Thirty micrograms of total cellular protein from each sample were resolved in a 10% SDS/PAGE, transferred, and immunoblotted with antibodies against p53 and mdm2. Please notice that in the Westerns blots shown here, the control lane (CTRL) has been moved from the first position to the second to facilitate the direct comparison of the control lane with the ADR lane to the right and the PTX 3 lane to the left.