Figure 1.
Viral transduction of PB-Calca neurons. A, Schematic of bilateral intracranial microinjection of Cre-dependent viruses encoding either mCherry or hM4Di:mCherry to the PB area in CalcaCre/+ mice. B, Microscope image of brain tissue showing Cre-dependent mCherry labeling of neurons in the external lateral parabrachial area (PBel). Nissl stain of an adjacent section is shown in B′. C–E, Microscope images showing Cre-dependent mCherry labeling of anterograde processes in the bed nucleus of the stria terminalis (BNST, panel C), central nucleus of the amygdala (CeA, panel D), and thalamus (panel E) that followed virus injection into the PB area in one CalcaCre/+ mouse. Nissl staining of adjacent sections are shown in C′–E′. Other abbreviations: Me5, mesencephalic trigeminal nucleus; me5, mesencephalic trigeminal tract; scp, superior cerebellar peduncle; 4/5 Cb, cerebellar lobule; LV, lateral ventricle; ac, anterior commissure; BLA, basolateral amygdaloid nucleus; 3V, third ventricle; fr, fasciculus retroflexus; VPPC, ventral posterior thalamic nucleus (parvicellular); mt, mammillothalamic tract.