Figure 3.
Effect of heat shock on levels of SOD1 in N2A cells. (A) Immunoblots of SOD1, human WT, G37R, or G41D-SOD1 permanently transfected mouse N2A cells were fractionated, electrophoresed (5 μg/lane), and examined as Fig. 1. (B) Mouse endogenous cytosolic and mitochondrial SOD1. Bands were quantitated by densitometry with National Institutes of Health IMAGE 1.62 software. The effect of heat shock (HS) on the ratios of mitochondrial/cytosolic mouse SOD1 is shown. (C) Human cytosolic (Cyt) and mitochondrial (Mit) SOD1 normalized to mouse endogenous cytosolic/mitochondrial SOD1. The effect of heat shock on the ratio of cytosolic/mitochondrial human WT, G37R, and G41D SOD1s is normalized to the corresponding ratios of mouse SOD1.