Figure 3.
Confocal microscopy of transfected COS-7 cells. Confocal microscopy of COS-7 cells 24 h after transfection with polyplexes of pCMVβ DNA and histone H2A; 10% of this DNA was FITC-labeled and 10% of this histone H2A was rhodamine-labeled. (A) Differential interference contrast (DIC) image of COS-7 cells. (B) The same cells as in A depicting the localization of FITC-labeled DNA transfected with polyplexes of fluorescein-labeled pCMVβ DNA. (C) The same cells as in A depicting the localization of rhodamine-labeled histone H2A. (D) The same cells as in A depicting the colocalization of FITC-labeled DNA and rhodamine-labeled DNA. The same cells as in A depicting the colocalization of FITC-labeled DNA and rhodamine-labeled DNA in both the cytoplasm and nucleus, suggesting that the DNA and histone H2A in these polyplexes not only forms a complex but is capable also of nuclear entry where the target gene can be transcribed.