TABLE 2.
Synthetic oligonucleotides used in this study
| Oligonucleotide | Sequencea |
|---|---|
| Oligonucleotides used for omcA experiments | |
| A5 | 5′-TTGATATCGGTGGCAGTGATGGRAAAGAT-3′ |
| A6 | 5′-TCAGTCGACTTAGTTACCGTGTGCTTCCAT-3′ |
| Oligonucleotides used for omcB experiments | |
| B1 | 5′-CATGTCGCCTTTAGTCACTTA-3′ |
| B2 | 5′-AACCAGTTTGCCAGCATCATT-3′ |
| B5 | 5′-GCAGTGGTAATAACGGCAATGAT-3′ |
| B6 | 5′-TCAACAACTGCACCGAAAGACTC-3′ |
| Oligonucleotides used for mtrA-mtrB experiments | |
| M1 | 5′-TACTGCCGGCACTTACCATCACA-3′ |
| M2 | 5′-GTGCGGTGTAGTCATGGCTGTTA-3′ |
| Oligonucleotides used for altA experiments | |
| C1 | 5′-TGGTCGACTTGGCTTTCTAGTGGGCTCAACC-3′ |
| C2 | 5′-TGGTCGACCCCGCTTGGCTGATGGAATACAC-3′ |
| C3 | 5′-CTGATCGATCGGCATTGACAAGAATAAT-3′ |
| C4 | 5′-CTGATCGATTCAGGCCAGAGTGAGACT-3′ |
| Oligonucleotides based on cat gene | |
| R1 | 5′-CAGACGGCATGATGAACCTGAATC-3′ |
| R2 | 5′-CCACCGTTGATATATCCCAATGGC-3′ |
| Oligonucleotide based on Kmr gene | |
| K1 | 5′-ATTGCGATCGATTTATGCTTGTAAACCGTT-3′ |
a
Underlined regions indicate the following restriction endonuclease sites engineered into the oligonulceotides: ClaI sites in C3, C4, and K1; SalI sites in C1, C2, and A6; and an EcoRV site in A5.