Fig. 5.
Inactivation of ospG induces a more rapid degradation of IκBα in infected epithelial cells. HeLa cells infected by derivatives of wild-type or ospG strains producing the AfaE adhesin were incubated at 25°C for 10 min to allow adhesion of bacteria to cells and then shifted to 37°C. At the indicated time points after temperature shift, cells were lysed and extracts were analyzed by SDS/PAGE and immunoblotting with anti-IκBα (a) and anti-phospho-IκBα (b) antibodies. A protein (indicated by X) recognized by the anti-IκBα serum was used as an internal loading control. NI, noninfected