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OspG inhibits activation of a NF-κB-regulated promoter. Luciferase and β-galactosidase activities were assayed after TNF-α stimulation of cells cotransfected with pIgγ-luc (encoding luciferase under the control of a NF-κB-regulated promoter), placZ (encoding β-galactosidase), pRK5myc, and either pRK5myc-OspG (w.t.) or pRK5myc-OspG-K53A (K53A) at the indicated amounts. Bars indicate fold inductions in luciferase activities normalized to β-galactosidase activities
