FIG. 3.
Loss of DUSP9/MKP-4 does not lead to significant changes in the basal phosphorylation state of MAPKs in placental tissues. (A) Representative sections from wild-type (WT) and null (KO) 10.5-dpc placentas stained using a polyclonal antiserum against DUSP9/MKP-4. The labyrinth layer is indicated by a bold line, and trophoblast giant cells (tgc) are arrowed. (B) Western blot analysis of protein lysates from two independent WT, KO, and heterozygous (Het) 10.5-dpc placentas. The protein species detected are indicated on the right, and tubulin is included (bottom) as a loading control. (C) Representative sections from WT and KO 10.5-dpc placentas stained using antibodies against the phosphorylated (active) forms of ERK1/2 (left) or p38 MAPK (right).