. 2005 Aug;43(8):3704–3712. doi: 10.1128/JCM.43.8.3704-3712.2005

TABLE 2.

Primers used for testing of LSPs

Sequence	PCR target	Forward primer	Reverse primer	Product size (bp)
LSP^P1	MAP0095	GCGATGTTCAAGAAGCCAGT	GGAGGTAATAGTCGCGTTCG	442
LSP^P2	MAP0284	CCAGTTACCTTCGACGAGGA	CTCTGTTGGATTCCCGTTTG	600
LSP^P3	MAP0388	GCAATCATCCCGACAACTG	TTCAGGTCGAGGTGTGACAA	166
LSP^P4	MAP0865	TCCTCTCCTTCGTCACCAAC	ATCTCAGACAGTGGCAGGT	597
LSP^P5	MAP0963	GCCACGAAGGACATCAATTC	GGTCGGGGTTGCTGATCTAT	592
LSP^P6	MAP1233	TCGTAACGTTAGCACCGAGA	AGCTTGAAGCATTCGACCAT	661
LSP^P7	MAP1345	GCACGATTCCGGTTCTTCTA	GCGGCGTTGAAATAGGTAGT	579
LSP^P8	MAP1633	AAAAGGGGAACGGTACAACC	ATTCACGCTGATCCTCGAAC	653
LSP^P9	MAP1718	TCCAATACAACCCGAAGACC	TTCCACGCTTTGGATAGACC	625
LSP^P10	MAP2027	CGACACCTCGGTGTTCATC	CCGAATAATCGCCAGGTTC	373
LSP^P11	MAP2154	GTCGGACCAGGGTTGAGAT	GGATAAGGCCGCATACAAAC	375
LSP^P12	MAP2181c	GCTTCGTCGGAATGTACGAG	AGCATCAGCCTGACTTGGTT	595
	MAP2182c	TCTGAACCCGGCTACACAC	CATGCCGGTGTGAGTACAA	430
	MAP2188c	GCCCGTAGAAGTGGACGAT	GATGACGGTCGCCAATCC	719
LSP^P13	MAP2765	ACTGATCCTGCCATGCCTAC	GCGTAACTCAGCGAACAACA	451
LSP^P14	MAP3726	GGGCTATCAGACGATGTTCC	TACCAGCAGTATCGCCGAAT	211
	MAP3750	CACTGAAGCTCGGCATGTAA	CGACGACGAGTCCTATCCAG	674
LSP^P15	MAP3774	AGCAGTGGACGAGGCAAC	GAGGGCGTAGAACTCTGTG	621
LSP^P16	MAP3815	GTAATGGCGCATTCTCTGGT	CCCTTGATCTCGACACTGGT	611
LSP^P17	MAP4269	GGAAACCAAGGAAGGGAAAG	GCTAGACGGGTGATCGTGTT	592
LSP^A8	Flanking sequence	TCTGCAGAGCGGTGACATC	ATACCGCCAACGACATCTTC	474
LSP^A8	Internal sequence	ACCGCCAGATGTTTCTCATC	GACTCGGTGCTGCTGGTC	166
LSP^A13	Flanking sequence^a	ACGAATGACGCCGTAGTAGC	GTCACGAGCGTCCATCCTAT	444
LSP^A13	Internal sequence	AGCTCAGAGGGCACTTTCCTT	CCAGGTCGATCAGGTCCTC	236
LSP^A14	Flanking sequence set A	ATATCGAGGCGTTCATCCTG	CACTCAACGAAGATCCAGCA	427
LSP^A14	Internal sequence	ATCACAGGTAGGAGCCTTGG	CACTTCAATGCCGAAATCAA	299
LSP^A14	Flanking sequence set B	GGCCTGGTATTCAACCTCA	CCGTTGACGTTGTGTTCAAG	718

The sequence of the reverse flanking primer used to amplify across LSP^A13 contains one SNP in comparison to the sequence of M. avium subsp. paratuberculosis strain K10.