Figure 3.
Inhibition of expression of a cell cycle control gene and a DNA methyltransferase. (A) Inhibition of CDK-2 expression. HeLa cells were transfected with either BS/U6 vector or BS/U6/cdk-2 and CMV-GFP to mark transfected cells as above. Solid arrows indicate two of the GFP-positive cells (transfected cells) in which CDK-2 expression was below the level of detection. Open arrows indicate two GFP-negative cells in which CDK-2 expression is also undetectable. (B) Inhibition of a DNA methyltransferase (DNMT-1) expression. HeLa cells were transfected with either BS/U6 vector or BS/U6/dnmt-1 and CMV-GFP to mark transfected cells as above. Solid arrows indicate two GFP-positive cells (transfected cells) in which DNMT-1 expression was barely detectable. Open arrows indicate two GFP-negative cells in which DNMT-1 expression is also undetectable. The latter are likely to have received the RNAi plasmid only, because of the excess ratio of the RNAi vector versus GFP-encoding plasmid used in transfection.