Figure 4.
Similar levels of telomerase activity are expressed in mTR+/+ and mTR+/− mice. Lysates were prepared from in vitro activated spleen cells derived from age-matched [(B6 mTR+/− × SPRET/Ei) × B6 mTR+/−] mice that were genotypically mTR+/+, mTR+/−, or mTR−/− and serially diluted before analyzing telomerase activity. Telomerase activity is shown for 4,000, 2,000, 1,000, and 500 cell equivalents. As a control for telomerase activity, a sample (4,000 cell equivalents) was heated to 85°C for 10 min. IS, the band produced by the internal standard included in the TRAP reaction.