Figure 2 |.
Validation of HemoLens’ components A) Temperature reading for both superfusion lines, bath (black), and vessel (grey) at water bath set point of 47C. Both lines reach physiological in 40 minutes and maintain this temperature with a stability of ± 0.32 °C over time. B) Micromanipulator highlighting movement axes. (i) Calculated linear movement for each axis. Full Turn: X: 0.985 mm ± 0.009 mm, Y: 0.996 mm ± 0.023 mm, Z: 0.982 ± (0.009). Quarter Turn: X: 0.247 ± 0.003 mm, Y: 0.250 ± 0.009 mm, Z: 0.244 mm ± 0.019 mm. n=10 for all axes. Data are represented as mean ± SEM C). incremental pressure ratchet system (iPRS). Inset showing internal stopping mechanisms. (i) measured increase per syringe tested, 3mL: 64.64 mmHg ± 2.94 mmHg, 1mL: 7.62 mmHg ± 0.194 mmHg. n=3 for each syringe types. Data are represented as mean ± SD D) Camera gantry system with call outs to important features. (i) Image showing rubber test tube with Vasotracker lines overlayed. (ii) Linear elastic response of the rubber test vessels with incremental pressure increases. R2 values of 0.98 and 0.99 for outer and inner diameters, respectively. E) RAMP system with call outs to important features. (i) Graphical representation of RAMP’s mechanism of action for cyclic pressure increases. F) Representative waveform of RAMP’s cyclic pressure generation set to physiological 80/120 mmHg at 65 beats per minute. (i) Average pressure increases per pulse from the base line, 48.16 mmHg ± 2.85 mmHg, n= 68. (ii) A single isolated square waveform with a spline fit superimposed demonstrating a similar curve to an arterial pulse waveform (inset). Arterial waveform adapted from Charlton et al 2019