Fig. 2.
Localization of FRC to Golgi units distinct from those containing SFL or RHO in imaginal disk cells. (A-C) Distinct localizations of FRC and SFL in Golgi units. Wing disks of wild-type larvae expressing MYC epitope-tagged FRC were stained with anti-MYC (red, arrows), anti-SFL (green, arrowheads), and anti-120-kDa protein (blue). The SFL signal was almost completely absent in sfl mutant embryos (data not shown), suggesting that the antibodies are specific for SFL. (D-F) Distinct localizations of FRC and RHO in Golgi units. Eye disks of wild-type larvae expressing FRC-MYC were stained with anti-MYC (red, arrows), anti-RHO (green, arrowheads), and anti-120-kDa protein. Some RHO staining did not overlap with that of the 120-kDa protein, as described (19). (Scale bars, 5 μm.)