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ALK3 is essential for cardiac myocyte survival. (a–h) Apoptosis was quantified by TUNEL analysis at E11.5 in the interventricular septum (a–d) and AV cushion (e–h). Green, TUNEL reaction; red, MF20 antibody to sarcomeric MHC (Texas red); blue, Hoechst dye 33258. (i and j) Mean ± SE for the interventricular septum (i) and endocardial cushion (j). − Cre, control embryos (ALK3F/−); + Cre, mutant embryos (ALK3F/−;αMHC-Cre+/−); *, P < 0.05.
