Figure 3.
Contribution of dynamin 1 to vesicle formation. (A) Western blot analysis of dynamin 1 immunoreactivity of control (Left) and dynamin-depleted (Right) brain cytosol. (B) Reduction of vesicle formation with dynamin-depleted cytosol. Liposomes were incubated with control or dynamin-depleted cytosol in the presence of ATP and GTP for 15 min at 37°C. Vesicle formation was detected by the DLS assay. The composition of liposomes was the same as that described for Fig. 1. Results were normalized to control values.