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. 2003 Aug 1;373(Pt 3):805–814. doi: 10.1042/BJ20030532

Characterization of SMOC-2, a modular extracellular calcium-binding protein.

Christian Vannahme 1, Silke Gösling 1, Mats Paulsson 1, Patrik Maurer 1, Ursula Hartmann 1
PMCID: PMC1223551  PMID: 12741954

Abstract

We have isolated the novel gene SMOC-2, which encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain found only in the homologous SMOC-1. Phylogenetic analysis of the calcium-binding domain sequences showed that SMOC-1 and -2 form a separate group within the BM-40 family. The human and mouse SMOC-2 sequences are coded for by genes consisting of 13 exons located on chromosomes 6 and 17, respectively. Analysis of recombinantly expressed protein showed that SMOC-2 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots and reverse transcription PCR revealed a widespread expression in many tissues.

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Selected References

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