Extended Data Table 1 |.
CryoEM data collection, processing, and model refinement statistics
| Data collection and processing | ||
|
| ||
| Magnification (nominal) | 130,000 | |
|
| ||
| Voltage (kV) | 300 | |
|
| ||
| Electron exposure (e−/Å2) | 63 | |
|
| ||
| Defocus range (μm) | 0.3-2.0 | |
|
| ||
| Pixel size (Å) | 0.843 | |
|
| ||
| Processing type | Global | Local |
|
| ||
| Symmetry imposed | C3 | none |
|
| ||
| Initial particle images (no.) | 381,075 | 497,199 |
|
| ||
| Final particle images (no.) | 165,733 | 209,164 |
|
| ||
| Map resolution (Å) | 2.6 | 3.2 |
| FSC threshold | 0.143 | 0.143 |
|
| ||
| Map sharpening B factor (Å2) | 85.7 | 114.8 |
|
| ||
|
| ||
| Refinement | ||
|
| ||
| Initial model used (PDB code) | 7LXY | 7SOB |
|
| ||
| Model resolution (Å) | 2.8 | 3.4 |
| FSC threshold | 0.5 | 0.5 |
|
| ||
| Model composition | ||
| Nonhydrogen atoms | 26,337 | 4006 |
| Protein residues | 3,768 | 496 |
| Glycan residues | 60 | 9 |
|
| ||
| B factors (Å2) | ||
| Protein | 34.9 | 17.0 |
| Glycans | 22.7 | 21.9 |
|
| ||
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.01 | 0.01 |
| Bond angles (°) | 1.03 | 1.03 |
|
| ||
|
| ||
| Validation | ||
|
| ||
| MolProbity score | 0.8 | 0.7 |
|
| ||
| Clashscore | 0.83 | 0.26 |
|
| ||
| Rotamer outliers (%) | 0.3 | 0.0 |
|
| ||
| Privateer glycan issues (%) | 0.0 | 0.0 |
|
| ||
| Ramachandran plot | ||
| Favored (%) | 98.0 | 97.5 |
| Allowed (%) | 2.0 | 2.5 |
| Outliers (%) | 0.0 | 0.0 |
|
| ||
| EMRinger score | 4.9 | 5.1 |
|
| ||
|
| ||
| Data Availability | ||
|
| ||
| EMDB | EMD-45174 | EMD-45175 |