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Effect of the constitutive expression of UEV-1 on the activity of the mitotic kinase cdk1 in HT-29-M6 cells. Synchronized control (left) and UEV-1-transfected (right) HT-29-M6 cells were analyzed for p13suc1-associated (cdk1) activity at 3-h intervals after release from the block at G1-S (top panel). Extracts from parallel cultures were analyzed by Western blotting for the subunit components of cdk1, p34cdc2 and cyclin B1, as indicated.
