FIG. 4.
The APC subunits Tsg24 and Cdc16 are differently located in mitotic cells. Mitotic Swiss-3T3 cells were fixed with methanol-acetone (50:50 [vol/vol]), triple stained with different combinations of antibodies, and analyzed by indirect immunofluorescence microscopy. Row 1 shows a metaphase cell labelled with the anti-Cdc16 antibody (1:150), a CREST antiserum (1:500), and Hoechst 33258. Row 2 shows a metaphase cell labelled with the anti-Tsg24 antibody (1:20), an antibody against centrosomal protein CTR453 (1:1), and Hoechst 33258. The secondary antibodies used were the same as for Fig. 1 and 2.