TABLE 1.
Yeast strains used in this study
| Strain | Genotype | Reference or comments |
|---|---|---|
| D150 | a ura3-52 leu2-3,112 ade1-100 his4-519 GAL+ | BWG1-7A, L. Guarente |
| YDL302 | As D150 with URA3 GAL10::dim1 | 26 |
| BMA38 | a/α ade2-1/ade2-1 can1-100/can1-100 his3Δ200/his3Δ200 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 | B. Dujon |
| YDL303 | As BM38 with dim1Δ::HIS3/DIM1 | Full DIM1 deletion made by PCR in strain BMA38 |
| YDL304A | a ade2-1 can1-100 his3Δ200 leu2-3,112 trp1-1 ura3-1 dim1Δ::HIS3 + pDL31.42 (URA3 2μm DIM1) | Starting strain, segregant from YDL303 containing a URA3 2μm DIM1 plasmid |
| YDL304B | α ade2-1 can1-100 his3Δ200 leu2-3,112 trp1-1 ura3-1 dim1Δ::HIS3 + pDL31.42 (URA3 2μm DIM1) | YDL304A sister strain |
| YDL209 | a ade2-1 can1-100 his3Δ200 leu2-3,112 trp1-1 ura3-1 dim1Δ::HIS3 + pTL6 (LEU2 ARS/CEN DIM1) | YDL304A in which plasmid pDL31.42 has been shuffled with plasmid pTL6 |
| MBS | a ade2 his3 leu2 trp1 ura3 can1 L-0 M-0 | P. Sarnow (17) |
| YDL321 | As MBS with dim1-2 | dim1-2 integrated as a dim1-2/HIS3 cassette isolated from pTL39 |
| YDL324 | As MBS with dim1-1 | dim1-1 integrated as a dim1-1/HIS3 cassette isolated from pTL43 |
| CH1462 | α ade2 ade3 his3 leu2 ura3 can1 | 21 |
| YDL314 | As CH1462 with dim1-9 | dim1-9 integrated as a dim1-9/HIS3 cassette isolated from pTL42 |
| YDL315 | As CH1462 with dim1-1 | dim1-1 integrated as a dim1-1/HIS3 cassette isolated from pTL43 |
| YDL300 | a/α ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre trp1Δ1/trp1Δ1 his3Δ200/his3Δ200 leu2Δ1/leu2Δ1 dim1Δ::URA3/DIM1 | 23 |
| YDL100A | a; as YDL300 + pTL6 (LEU2 ARS/CEN DIM1) | dim1Δ::URA3 segregant from YDL300 |
| YDL101A | a; as YDL300 + pTL18 (LEU2 ARS/CEN 3× Myc-Dim1p) | dim1Δ::URA3 segregant from YDL300 |
| YDL102A | a; as YDL300 + pTL25 (LEU2 ARS/CEN Dim1p-3× Myc) | dim1Δ::URA3 segregant from YDL300 |