Abstract
We have sampled a London population of Drosophila melanogaster for null alleles at twenty-five allozyme loci. The same loci and biochemical techniques were used as in our previous survey of a North Carolina population (Voelker et al. 1980). This second survey is completely concordant with the first. No nulls were detected among the five X-linked loci. The mean frequency of nulls at the twenty autosomal loci was 0.0023. Although there is significant interlocus heterogeneity, the two populations appear to have the same frequencies at each locus. This suggests that null alleles at these allozyme loci are in mutation-selection balance, and we estimate the average heterozygous effect of an allozyme null to be 0.0015. Consideration of allozyme null-allele frequencies, the effects of allozyme null alleles on viability and fertility and the generally greater amount of genetic variability at allozyme loci determined by electrophoresis lead us to doubt the validity of generalizing from allozyme data to the whole genome.
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Selected References
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- Mukai T., Yamaguchi O. The genetic structure of natural populations of Drosophila melanogaster. XI. Genetic variability in a local population. Genetics. 1974 Feb;76(2):339–366. doi: 10.1093/genetics/76.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Racine R. R., Langley C. H. Genetic heterozygosity in a natural population of Mus musculus assessed using two-dimensional electrophoresis. Nature. 1980 Feb 28;283(5750):855–857. doi: 10.1038/283855a0. [DOI] [PubMed] [Google Scholar]
- Smith S. C., Racine R. R., Langley C. H. Lack of genic variation in the abundant proteins of human kidney. Genetics. 1980 Dec;96(4):967–974. doi: 10.1093/genetics/96.4.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sturtevant A. H. High Mutation Frequency Induced by Hybridization. Proc Natl Acad Sci U S A. 1939 Jul;25(7):308–310. doi: 10.1073/pnas.25.7.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Voelker R. A., Schaffer H. E., Mukai T. Spontaneous Allozyme Mutations in DROSOPHILA MELANOGASTER: Rate of Occurrence and Nature of the Mutants. Genetics. 1980 Apr;94(4):961–968. doi: 10.1093/genetics/94.4.961. [DOI] [PMC free article] [PubMed] [Google Scholar]