Abstract
Three new 9-aminoacridine (9AA) resistant mutations of bacteriophage T4D have been isolated and characterized. Two of the mutations, rs and rc, have identical patterns of acridine resistance, but they map on opposite sides of the rII region. In addition, rs has an effect on the plaque morphology of r mutations, whereas rc does not. The third mutation, ama, maps very close to rs but exhibits a different pattern of resistance to 9AA. None of the three is resistant to acridines by virtue of reduced permeability. Taken together with other mutations that have been previously characterized, these new mutations permit us to set the minimum number of acridine-sensitive processes in T4 development at four.
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Selected References
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- Altman S., Lerman L. S. Kinetics and intermediates in the intracellular synthesis of bacteriophage T4 deoxyribonucleic acid. J Mol Biol. 1970 Jun 14;50(2):235–261. doi: 10.1016/0022-2836(70)90190-7. [DOI] [PubMed] [Google Scholar]
- Couse N. L. Control of lysis of T4-infected Escherichia coli. J Virol. 1968 Mar;2(3):198–207. doi: 10.1128/jvi.2.3.198-207.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE MARS R. I. The production of phage-related materials when bacteriophage development in interrupted by proflavine. Virology. 1955 May;1(1):83–99. doi: 10.1016/0042-6822(55)90007-6. [DOI] [PubMed] [Google Scholar]
- DOERMANN A. H. The intracellular growth of bacteriophages. I. Liberation of intracellular bacteriophage T4 by premature lysis with another phage or with cyanide. J Gen Physiol. 1952 Mar;35(4):645–656. doi: 10.1085/jgp.35.4.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDGAR R. S., EPSTEIN R. H. Inactivation by ultraviolet light of an acriflavine-sensitive gene function in phage T4D. Science. 1961 Aug 4;134(3475):327–328. doi: 10.1126/science.134.3475.327. [DOI] [PubMed] [Google Scholar]
- Foster R. A. An Analysis of the Action of Proflavine on Bacteriophage Growth. J Bacteriol. 1948 Dec;56(6):795–809. doi: 10.1128/jb.56.6.795-809.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hessler A. Y. Acridine resistance in bacteriophage T2H as a function of dye penetration measured by mutagenesis and photoinactivation. Genetics. 1965 Oct;52(4):711–722. doi: 10.1093/genetics/52.4.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAUFMAN E., HIATT C. W. Photodynamic action of proflavine on T2 coliphage. Virology. 1959 Nov;9:478–479. doi: 10.1016/0042-6822(59)90136-9. [DOI] [PubMed] [Google Scholar]
- Mattson T. L. Recombination of bacteriophage T4 stimulated by 9-aminoacridine. Genetics. 1970 Aug;65(4):535–544. doi: 10.1093/genetics/65.4.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SILVER S. ACRIFLAVINE RESISTANCE: A BACTERIOPHAGE MUTATION AFFECTING THE UPTAKE OF DYE BY THE INFECTED BACTERIAL CELLS. Proc Natl Acad Sci U S A. 1965 Jan;53:24–30. doi: 10.1073/pnas.53.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STEINBERG C. M., EDGAR R. S. A critical test of a current theory of genetic recombination in bacteriophage. Genetics. 1962 Feb;47:187–208. doi: 10.1093/genetics/47.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUSMAN M., PIECHOWSKI M. M. STUDIES ON PHAGE DEVELOPMENT. I. AN ACRIDINE-SENSITIVE CLOCK. Virology. 1965 Jun;26:163–174. doi: 10.1016/0042-6822(65)90043-7. [DOI] [PubMed] [Google Scholar]