FIG. 4.
Cellular distribution of RSV Fopt in nonpolarized epithelial cells. (a) Schematic representation of full-length and mutant forms of RSV Fopt. (b to e) HEp-2 cells were transfected with each RSV Fopt construct noted in panel a to assess the cellular distribution of each protein. (b) RSV infection; (c) Fopt; (d) Fopt(TM + 1); (e) Fopt(ΔCT). Transfected cells were fixed, permeabilized, immunolabeled, and analyzed by confocal microscopy 48 h after transfection; RSV-infected HEp-2 cells were analyzed in an analogous manner and are shown for comparison. Only the first residue of the Fopt CT was required to achieve plasma membrane localization.