Figure 3:
Tissue Yield and Viability Expectations. A) Grams of indicated lymph nodes collected per donor.
(B) Percentage of live cells recovered for indicated tissue per donor.
(C) Number of viable cells recovered per mL [BLD,BOM] or gram [all others] of tissue per donor. (D) Frequency of T cells per tissue representative of each protocol.
Box and Whisker indicate mean with 95% confidence interval. Tissue Abbreviations: BLD - Blood, BOM - Bone Marrow, SPL - Spleen, ILN - Iliac Lymph Nodes (LNs), CLN - Colonic LNs, LLN - Lung LNs, HLN - Hepatic LNs, MLN - Mesenteric LNs, BAL - Bronchioalveolar Lavage, LNG - Lung, JEL - Jejunum Epithelial Layer (EL), JLP - Jejunum Lamina Propria (LP), IEL - Illeum EL, ILP - Illeum LP, aCEL - ascending Colonic EL, aCLP – ascending Colonic LP, dCEL - descending Colonic EL, dCLP – descending Colonic LP, LVR - Liver. Color Coding: Red - Protocol 1, Yellow - Protocol 2, Blue - Protocol 3, Green - Protocol 4, Orange - Protocol 5. Data (A-C) are pooled from 185 unique donors ranging from 19–185 donors per tissue. Data (D) are pooled from 5–46 donors.