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. 1985 Jun;110(2):187–216. doi: 10.1093/genetics/110.2.187

The Role of the SPO11 Gene in Meiotic Recombination in Yeast

Sue Klapholz 1, Candace S Waddell 1, Rochelle Easton Esposito 1
PMCID: PMC1202559  PMID: 3891509

Abstract

Several complementary experimental approaches were used to demonstrate that the SPO11 gene is specifically required for meiotic recombination. First, sporulating cultures of spo11-1 mutant diploids were examined for landmark biochemical, cytological and genetic events of meiosis and ascosporogenesis. Cells entered sporulation with high efficiency and showed a near-doubling of DNA content. Synaptonemal complexes, hallmarks of intimate homologous pairing, and polycomplex structures appeared during meiotic prophase. Although spontaneous mitotic intra- and intergenic recombination occurred at normal levels, no meiotic recombination was observed. Whereas greater than 50% of cells completed both meiotic divisions, packaging of the four meiotic products into mature ascospores took place in only a small subset of asci. Haploidization occurred in less than 1% of viable colony-forming units. Second, the Rec- meiotic defect conferred by spo11-1 was confirmed by dyad analysis of spores derived from spo13-1 single-division meiosis in which recombination is not a requirement for viable ascospore production. Diploids homozygous for the spo13-1 mutation undergo meiotic levels of exchange followed by a single predominantly equational division and form asci containing two near-diploid spores. With the introduction of the spo11-1 mutation, high spore viability was retained, whereas intergenic recombination was reduced by more than 100-fold.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker B. S., Carpenter A. T., Esposito M. S., Esposito R. E., Sandler L. The genetic control of meiosis. Annu Rev Genet. 1976;10:53–134. doi: 10.1146/annurev.ge.10.120176.000413. [DOI] [PubMed] [Google Scholar]
  2. Bruschi C. V., Esposito M. S. Enhancement of spontaneous mitotic recombination by the meiotic mutant spo11-1 in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7566–7570. doi: 10.1073/pnas.80.24.7566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davidow L. S., Goetsch L., Byers B. Preferential Occurrence of Nonsister Spores in Two-Spored Asci of SACCHAROMYCES CEREVISIAE: Evidence for Regulation of Spore-Wall Formation by the Spindle Pole Body. Genetics. 1980 Mar;94(3):581–595. doi: 10.1093/genetics/94.3.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Golubovskaya I. N. Genetic control of meiosis. Int Rev Cytol. 1979;58:247–290. doi: 10.1016/s0074-7696(08)61477-1. [DOI] [PubMed] [Google Scholar]
  5. Horesh O., Simchen G., Friedmann A. Morphogenesis of the synapton during yeast meiosis. Chromosoma. 1979 Oct 2;75(1):101–115. doi: 10.1007/BF00330628. [DOI] [PubMed] [Google Scholar]
  6. Klapholz S., Esposito R. E. Isolation of SPO12-1 and SPO13-1 from a natural variant of yeast that undergoes a single meiotic division. Genetics. 1980 Nov;96(3):567–588. doi: 10.1093/genetics/96.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Liras P., McCusker J., Mascioli S., Haber J. E. Characterization of a mutation in yeast causing nonrandom chromosome loss during mitosis. Genetics. 1978 Apr;88(4 Pt 1):651–671. [PMC free article] [PubMed] [Google Scholar]
  8. Malone R. E., Esposito R. E. Recombinationless meiosis in Saccharomyces cerevisiae. Mol Cell Biol. 1981 Oct;1(10):891–901. doi: 10.1128/mcb.1.10.891-901.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moens P. B., Esposito R. E., Esposito M. S. Aberrant nuclear behavior at meiosis and anucleate spore formation by sporulation-deficient (SPO) mutants of Saccharomyces cerevisiae. Exp Cell Res. 1974 Jan;83(1):166–174. doi: 10.1016/0014-4827(74)90700-9. [DOI] [PubMed] [Google Scholar]
  10. Moens P. B., Mowat M., Esposito M. S., Esposito R. E. Meiosis in a temperature-sensitive DNA-synthesis mutant and in an apomictic yeast strain (Saccharomyces cerevisiae). Philos Trans R Soc Lond B Biol Sci. 1977 Mar 21;277(955):351–358. doi: 10.1098/rstb.1977.0023. [DOI] [PubMed] [Google Scholar]
  11. Mortimer R. K., Schild D. Genetic map of Saccharomyces cerevisiae. Microbiol Rev. 1980 Dec;44(4):519–571. doi: 10.1128/mr.44.4.519-571.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olson L. W., Zimmermann F. K. Meiotic recombination and synaptonemal complexes in Saccharomyces cerevisiae. Mol Gen Genet. 1978 Oct 30;166(2):151–159. doi: 10.1007/BF00285917. [DOI] [PubMed] [Google Scholar]
  13. Roman H, Phillips M M, Sands S M. Studies of Polyploid Saccharomyces. I. Tetraploid Segregation. Genetics. 1955 Jul;40(4):546–561. doi: 10.1093/genetics/40.4.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rothstein R. J., Esposito R. E., Esposito M. S. The effect of ochre suppression on meiosis and ascospore formation in Saccharomyces. Genetics. 1977 Jan;85(1):35–54. doi: 10.1093/genetics/85.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sherman F. Use of micromanipulators in yeast studies. Methods Cell Biol. 1975;11:189–199. doi: 10.1016/s0091-679x(08)60323-4. [DOI] [PubMed] [Google Scholar]
  16. Wagstaff J. E., Klapholz S., Esposito R. E. Meiosis in haploid yeast. Proc Natl Acad Sci U S A. 1982 May;79(9):2986–2990. doi: 10.1073/pnas.79.9.2986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Westergaard M., von Wettstein D. The synaptinemal complex. Annu Rev Genet. 1972;6:71–110. doi: 10.1146/annurev.ge.06.120172.000443. [DOI] [PubMed] [Google Scholar]
  18. Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. doi: 10.1016/s0091-679x(08)60963-2. [DOI] [PubMed] [Google Scholar]
  19. Zickler D., Olson L. W. The synaptonemal complex and the spindle plaque during meiosis in yeast. Chromosoma. 1975;50(1):1–23. doi: 10.1007/BF00284959. [DOI] [PubMed] [Google Scholar]

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