FIG. 2.
Immunodetection of the LytC lysozyme in the cell envelope of the TIGR4 strain. (A) Western blot analysis of cell extracts prepared from the following strains (indicated at the top): M31 (ΔlytA), M31C (ΔlytA lytC::ermC), R6 (wild type), R6C (R6 but lytC::ermC), TIGR4 (capsular serotype 4 strain), and P036 (TIGR4 but lytC::tet). The Mrs of the size standards (S) are indicated on the left. (B) The double-immunodiffusion experiment was performed on an agarose-coated microscope slide with anti-LytC antiserum in the center well (7) and cell envelope preparations from M31, P026 (TIGR4 but lytA::kan), P031 (TIGR4 but lytA::kan lytC::tet), and M31C in wells 1, 2, 3, and 6, respectively. In wells 4 and 5, phosphate-buffered saline and 1 μg of pure LytC, respectively, were used.