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. 2025 Jan 30;6(2):101931. doi: 10.1016/j.xcrm.2025.101931
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-hCD3-PE BioLegend Cat#300308; RRID: AB_314043
Anti-hCD8-PerCP BioLegend Cat#301030; RRID: AB_893425
Anti-hγδTCR-APC BioLegend Cat#331212; RRID: AB_1089215
Anti-h/mCD11b-APC/Cy7 BioLegend Cat#101225; RRID: AB_830641
Anti-hHLA-A,B,C-APC BioLegend Cat#311417; RRID: AB_493669
Anti-mNKG2D-APC BioLegend Cat#130211; RRID: AB_1236372
Anti-mCD45.1-AF488 BioLegend Cat#110718; RRID: AB_492862
Anti-mCD45.2-PE BioLegend Cat#109807; RRID: AB_313444
Anti-mCD11b-BV605 BioLegend Cat#101257; RRID: AB_11126744
Anti-mCD206-APC BioLegend Cat#141708; RRID: AB_10896057
Anti-mCD86-PE BioLegend Cat#305405; RRID: AB_314525
Anti-mCD86-BV650 BioLegend Cat#105036; RRID: AB_11126147
Anti-mH2-Db-APC BioLegend Cat#111514; RRID: AB_2565862
Anti-mCD335-BV510 BioLegend Cat#137623; RRID: AB_2563290
Anti-mCD11b-BV605 BioLegend Cat#101257; RRID: AB_11126744
Anti-mCD206-PE/Cy7 BioLegend Cat#141719; RRID: AB_2562247
Anti-mCD45.2-PE/Dazzle BioLegend Cat#109845; RRID: AB_2564176
Anti-mNKG2D-APC BioLegend Cat#130211; RRID: AB_1236372
Anti-hCD4-FITC BD Biosciences Cat#555346; RRID: AB_395751
Anti-hCD56-APC BD Biosciences Cat#555518; RRID: AB_398601
Anti-mCD8-BV786 BD Biosciences Cat#563332; RRID: AB_2721167
Anti-mRae1-BV711 BD Biosciences Cat#748077; RRID: AB_2872538
Anti-mCD3-PerCP/Cy5.5 BD Biosciences Cat#560527; RRID: AB_1727463
Anti-mCD335-PE BD Biosciences Cat#560757; RRID: AB_1727466
Anti-hCD34(RQR8)-PE Thermo Fisher Scientific Cat#MA1-10205; RRID: AB_11152571
Anti-hMICA/MICB eBioscience Cat#12-5788-42; RRID: AB_10854117
Anti-hNKG2D-APC eBioscience Cat#17-5878-81; RRID: AB_469462
Anti-hS100B-AF555 Abcam Cat#ab215989
Anti-hNestin-PE BioLegend Cat#656806; RRID: AB_2566381
Anti-hCD45-AF647 BioLegend Cat#368538; RRID: AB_2716028
Anti-hEndomucin (V.7C7) Santa Cruz Biotechnology Cat#Sc-65495
Donkey-anti-rat AF647 Thermo Fisher Scientific Cat#A48272; RRID: AB_2893138
InVivoMAb anti-mouse CD3ε (clone 145-2C11) BioXCell Cat#BE0001-1; RRID: AB_1107634
InVivoMAb anti-mouse CD28 (clone 37.51) BioXCell Cat#BE0015-1; RRID: AB_1107624
Bacterial and virus strains
Retrovirus MSGV-1D3-28Z Addgene Cat#107226
Biological samples
Human glioblastoma samples Neurosurgery Department of the University Hospital of Zurich https://www.usz.ch/en/department/neurosurgery/
Chemicals, peptides, and recombinant proteins
Recombinant Murine IL-2 Peprotech Cat#212-12
Recombinant Murine IL-15 Peprotech Cat#210-15
Recombinant Human IFN-γ Peprotech Cat#300-02
Recombinant Human TGF-β1 (HEK293 derived) Peprotech Cat#100-21
Recombinant Human TGF-β2 (HEK293 derived) Peprotech Cat#100-35B
Animal-Free Recombinant Human EGF Peprotech Cat#AF-100-15-1
Recombinant Human FGF-basic (154 a.a.) Peprotech Cat#100-18B
Recombinant Human M-CSF (carrier-free) Biolegend Cat#574804
Recombinant Human GM-CSF (carrier-free) Biolegend Cat#572904
Dynabeads™ Human T-Activator CD3/CD28 for T cell Expansion and Activation Thermo Fisher Scientific Cat#11131D
Formaldehyde 16% Sol. em Grade 10x10mL Electron Microscopy Sciences Cat#15710
ACK lysis buffer Thermo Fisher Scientific Cat#A1049201
Percoll Sigma-Aldrich Cat#17-0891-02
Collagenase from clostridium histolyticum Sigma-Aldrich Cat#C5138-1G
Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat#DN25-100MG
Retro-Concentin Systems Biosciences Cat#SBI-RV100A-1
RapiClear 1.52 Sunjin Lab Cat#RC152002:100mL
Fugene 6 transfection reagent Promega Cat#E2693
RetroNectin® Recombinant Human Fibronectin Fragment Takara Cat#T100B
Polybrene Santa Cruz Cat#sc-134220
Critical commercial assays
EasySep Mouse NK Cell Isolation Kit STEMCELL Technologies Cat#19855
EasySep™ Release Human CD3 Positive Selection Kit STEMCELL Technologies Cat#17751
Pan T cell Isolation Kit II, mouse Miltenyi Biotec Cat#130-095-130
EasySep™ Human CD14 Positive Selection Kit II STEMCELL Technologies Cat#17858
PKH26 Red Fluorescent Cell Linker Kit Sigma-Aldrich Cat#PKH26GL-1KT
Zombie Violet Fixable Viability Kit Biolegend Cat#423114
Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, 4 rxns 10x Genomics Cat#PN-1000128
3′ CellPlex Kit 10x Genomics Cat#PN-1000261
IFN gamma Mouse Uncoated ELISA Kit with Plates Thermo Fisher Scientific Cat#88-7314-22
Deposited data
scRNA-seq data This paper GEO: GSE283049
scRNA-seq code This paper Zenodo: https://doi.org/10.5281/zenodo.14230743
Experimental models: Cell lines
LN-229 Dr. N. de Tribolet N/A
ZH-161 Neurology Department of the University Hospital of Zurich N/A
CT-2A Merck Cat#SCC194
GL-261 National Cancer Institute N/A
SB-28 Leibniz-Institute German Collection of Microorganisms and Cell Cultures (DSMZ) Cat#ACC 880
L929 Dr. Tomasz Rygiel at the Department of Immunology, Medical University of Warsaw N/A
E0771-BrM Dr. Manuel Valiente at the Spanish National Cancer Research Center (CNIO) of Madrid N/A
LLC-BrM Dr. Manuel Valiente at the Spanish National Cancer Research Center (CNIO) of Madrid N/A
Platinum-E (Plat-E) Retroviral Packaging Cell Line Cell Biolabs N/A
K562-mbIL21 feeder cells Dr. Obinna Chijioke at Institute of Experimental Immunology of University of Zurich N/A
A20 Dr. Christian Pellegrino at Department of Medical Oncology and Hematology of University Hospital Zurich N/A
Experimental models: Organisms/strains
C57BL/6J Janvier Labs Cat#SC-C57N-F
C57BL/6J 45.1 Own breeding N/A
Software and algorithms
CellProfiler (2.2.0 and 4.2.1) https://cellprofiler.org/ N/A
FlowJo (Tree Star) https://www.flowjo.com/solutions/flowjo N/A
GraphPad https://www.graphpad.com/features N/A
CellRanger version 7.1.0 https://www.10xgenomics.com/support/software/cell-ranger/latest N/A
R version 4.3.1 https://www.r-project.org/ N/A
scRNA-seq code This paper Zenodo: https://doi.org/10.5281/zenodo.14230743
Other
96-well ViewPlates PerkinElmer Cat#6005182
Corning®-Zellsieb pore size 70 μm Sigma-Aldrich Cat#CLS431751