Figure 2. Cross-linking of MAP1B with LIS1.
Protein from a mouse brain extract was incubated for 30 min at room temperature in the presence or absence of formaldehyde (FA). After this incubation, the protein was separated by gel electrophoresis and characterized by Western blotting (WB) using an antibody raised against MAP1B or LIS1. Additionally, the amount of actin in each sample was determined by dot blot (DB). The electrophoretic mobilities for MAP1B and LIS1 as well as the 200 and 40 kDa markers are indicated.