. Author manuscript; available in PMC: 2025 Jan 19.

Published in final edited form as: Cytometry A. 2010 May;77(5):410–419. doi: 10.1002/cyto.a.20859

Table 1.

Panel description (A) and compensation matrix (B)

(A) PANEL DESCRIPTION

PANEL	FITC	PE	TEXAS RED-PE	CY5-PE	CY5.5-PERCP	CY7-PE	APC	ALEXA 700	CY7-APC	PACIFIC BLUE	AMCYAN	QDOT655

12-Colors	CD16	CD141	CD34	CD11c	CD123	CD20+CD56	CD1c	CD3	HLA-DR	CD14	CD4	CD8
Lymphocyte	CD16	-	-	-	-	CD56	-	CD3	CD20	-	CD4	CD8
Monocyte	CD16	-	-	-	-	CD20	-	CD3	HLA-DR	CD14	-	-
DC	CD16	CD141	CD34	CD11c	CD123	CD3+CD14+CD20+CD56	CD1c	-	HLA-DR	-	-	-
(B) COMPENSATION MATRIX

	FITC	PE	TEXAS RED-PE	CY5-PE	CY5.5-PERCP	CY7-PE	APC	ALEXA 700	CY7-APC	PACIFIC BLUE	AMCYAN	QDOT 655

FITC		19.67	16.76	5.18	2.14	0.41	0	0	0	0	2.78	0.20
PE	0.69		88.63	30.55	13.66	2.29	0.11	0	0	0	0.1	2.04
Texas Red-PE	0.08	4.32		42.32	24.77	5.26	1.09	0.59	0.09	0	0	2.74
Cy5-PE	0	0.85	1.05		80.78	19.63	74.13	47.02	8.66	0	0	4.98
Cy5.5-PerCP	0	0	0	26.14		29.51	5.23	49.58	10.41	0.39	0.13	11.62
Cy7-PE	0.06	3.46	3.07	1.16	1.24		0.08	0.07	4.94	0	0	0.05
APC	0	0	0	0.74	0.31	0.07		28.8	6.29	0	0	1.95
Alexa 700	0.05	0	0.07	0.07	2.08	0.88	0.73		32.54	0	0	0.06
Cy7-APC	0	0	0	0.13	0.06	1.96	14.03	5.13		0	0	0.26
Pacific Blue	0	0	0	0	0	0	0	0	0		11.94	0.43
AmCyan	56.21	8.36	6.59	1.13	0.85	0.57	0.10	−0.53	0.75	10.27		3.20
QDot 655	0	0	4.08	77.4	4.95	0.08	53.12	2.67	0	0	0

Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin; Cy5, Cyanine-5; Cy5.5-PerCP, Cyanine-5.5-peridin-chlorophyll; APC, allophycocyanin; QDot, Quantum dot.

Compensation matrix obtained by staining Antibody-capture beads Compbeads with each of the mAbs used in the panel, with the exception of Alexa 700-anti-CD3 used on cells. Spectral overlap or spillover between fluorochromes was calculated using flowJo software 8.7.1.