Figure 3.
Ssh1p interacts with the signal sequences of invertase and Kar2p. Yeast cells that contained different signal sequence bearing Cub-Ura3p and Nub-Sec61p, Nub-Ssh1p, Nub-Sec62p, or Nub-Bos1p were grown to an OD600 of 1. Then 4 μl of two serial 1:10 dilutions were spotted onto media lacking uracil and tryptophan and histidine to select for the presence of the plasmids expressing the fusion proteins. Growth was recorded after 3 d at 30°C. The growth of the cells is a measure of the interaction between the signal sequence of the Cub-Ura3p and the cotransformed Nub-fusion protein.