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. 1974 Nov;143(2):403–409. doi: 10.1042/bj1430403

Deoxycytidylate deaminase. Evidence for a new enzyme in cells infected by the virus of herpes simplex

Hilary A Rolton 1,*, Hamish M Keir 1
PMCID: PMC1168395  PMID: 4376945

Abstract

The activities of dCMP deaminase and DNA polymerase I increased twofold and fivefold in BHK-21/C13 cells after infection by the virus of herpes simplex. The increases were greatly diminished, and under certain conditions prevented, by inclusion of actinomycin D or cycloheximide in the cell–virus system during the infective cycle. The dCMP deaminase purified from infected cells harvested 8h after infection differed from the deaminase purified from non-infected cells inasmuch as (a) it was more resistant to heating at 37°C; (b) the substrate (dCMP) concentration at half-maximum velocity was lower; (c) maximum activation was achieved by a lower concentration of dCTP; (d) it was more resistant to inhibition by dTTP; and (e) it behaved differently when assayed in the presence of a herpes-virus-specific antiserum. The DNA polymerase activity in the infected cells was markedly decreased in the presence of the herpes-virus-specific antiserum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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