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. 1986 Feb;5(2):381–386. doi: 10.1002/j.1460-2075.1986.tb04223.x

Functional homology between the sequence-specific DNA-binding proteins nuclear factor I from HeLa cells and the TGGCA protein from chicken liver.

P A Leegwater, P C van der Vliet, R A Rupp, J Nowock, A E Sippel
PMCID: PMC1166743  PMID: 3709517

Abstract

Nuclear factor I from HeLa cells, a protein with enhancing function in adenovirus DNA replication, and the chicken TGGCA protein are specific DNA-binding proteins that were first detected by independent methods and that appeared to have similar DNA sequence specificity. To test whether they are homologous proteins from different species we have compared (i) their DNA binding properties and (ii) their function in reconstituted adenovirus DNA replication systems. Using deletion and substitution mutants derived from the DNA binding site on the adenovirus 2 inverted terminal repeat, it was found that the two proteins protect the same 24-nucleotide region of both strands against DNase I digestion and that they have identical minimal recognition sequences of 15 bp containing dyad symmetry. Like nuclear factor I, the TGGCA protein enhances the initiation reaction of adenovirus 2 DNA replication in vitro in a DNA recognition site-dependent manner.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aleström P., Stenlund A., Li P., Pettersson U. A common sequence in the inverted terminal repetitions of human and avian adenoviruses. Gene. 1982 May;18(2):193–197. doi: 10.1016/0378-1119(82)90117-2. [DOI] [PubMed] [Google Scholar]
  2. Borgmeyer U., Nowock J., Sippel A. E. The TGGCA-binding protein: a eukaryotic nuclear protein recognizing a symmetrical sequence on double-stranded linear DNA. Nucleic Acids Res. 1984 May 25;12(10):4295–4311. doi: 10.1093/nar/12.10.4295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cornish-Bowden A. Nomenclature for incompletely specified bases in nucleic acid sequences: recommendations 1984. Nucleic Acids Res. 1985 May 10;13(9):3021–3030. doi: 10.1093/nar/13.9.3021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gronostajski R. M., Adhya S., Nagata K., Guggenheimer R. A., Hurwitz J. Site-specific DNA binding of nuclear factor I: analyses of cellular binding sites. Mol Cell Biol. 1985 May;5(5):964–971. doi: 10.1128/mcb.5.5.964-971.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guggenheimer R. A., Nagata K., Lindenbaum J., Hurwitz J. Protein-primed replication of plasmids containing the terminus of the adenovirus genome. I. Characterization of an in vitro DNA replication system dependent on adenoviral DNA sequences. J Biol Chem. 1984 Jun 25;259(12):7807–7814. [PubMed] [Google Scholar]
  6. Guggenheimer R. A., Stillman B. W., Nagata K., Tamanoi F., Hurwitz J. DNA sequences required for the in vitro replication of adenovirus DNA. Proc Natl Acad Sci U S A. 1984 May;81(10):3069–3073. doi: 10.1073/pnas.81.10.3069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hay R. T. The origin of adenovirus DNA replication: minimal DNA sequence requirement in vivo. EMBO J. 1985 Feb;4(2):421–426. doi: 10.1002/j.1460-2075.1985.tb03645.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hennighausen L., Siebenlist U., Danner D., Leder P., Rawlins D., Rosenfeld P., Kelly T., Jr High-affinity binding site for a specific nuclear protein in the human IgM gene. Nature. 1985 Mar 21;314(6008):289–292. doi: 10.1038/314289a0. [DOI] [PubMed] [Google Scholar]
  9. Lally C., Dörper T., Gröger W., Antoine G., Winnacker E. L. A size analysis of the adenovirus replicon. EMBO J. 1984 Feb;3(2):333–337. doi: 10.1002/j.1460-2075.1984.tb01807.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leegwater P. A., van Driel W., van der Vliet P. C. Recognition site of nuclear factor I, a sequence-specific DNA-binding protein from HeLa cells that stimulates adenovirus DNA replication. EMBO J. 1985 Jun;4(6):1515–1521. doi: 10.1002/j.1460-2075.1985.tb03811.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nagata K., Guggenheimer R. A., Enomoto T., Lichy J. H., Hurwitz J. Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6438–6442. doi: 10.1073/pnas.79.21.6438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nagata K., Guggenheimer R. A., Hurwitz J. Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6177–6181. doi: 10.1073/pnas.80.20.6177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nowock J., Borgmeyer U., Püschel A. W., Rupp R. A., Sippel A. E. The TGGCA protein binds to the MMTV-LTR, the adenovirus origin of replication, and the BK virus enhancer. Nucleic Acids Res. 1985 Mar 25;13(6):2045–2061. doi: 10.1093/nar/13.6.2045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nowock J., Sippel A. E. Specific protein-DNA interaction at four sites flanking the chicken lysozyme gene. Cell. 1982 Sep;30(2):607–615. doi: 10.1016/0092-8674(82)90257-4. [DOI] [PubMed] [Google Scholar]
  15. Pearson G. D., Chow K. C., Enns R. E., Ahern K. G., Corden J. L., Harpst J. A. In vitro replication directed by a cloned adenovirus origin. Gene. 1983 Sep;23(3):293–305. doi: 10.1016/0378-1119(83)90019-7. [DOI] [PubMed] [Google Scholar]
  16. Rawlins D. R., Rosenfeld P. J., Wides R. J., Challberg M. D., Kelly T. J., Jr Structure and function of the adenovirus origin of replication. Cell. 1984 May;37(1):309–319. doi: 10.1016/0092-8674(84)90327-1. [DOI] [PubMed] [Google Scholar]
  17. Shinagawa M., Ishiyama T., Padmanabhan R., Fujinaga K., Kamada M., Sato G. Comparative sequence analysis of the inverted terminal repetition in the genomes of animal and avian adenoviruses. Virology. 1983 Mar;125(2):491–495. doi: 10.1016/0042-6822(83)90221-0. [DOI] [PubMed] [Google Scholar]
  18. Siebenlist U., Hennighausen L., Battey J., Leder P. Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma. Cell. 1984 Jun;37(2):381–391. doi: 10.1016/0092-8674(84)90368-4. [DOI] [PubMed] [Google Scholar]
  19. Stillman B. W. The replication of adenovirus DNA with purified proteins. Cell. 1983 Nov;35(1):7–9. doi: 10.1016/0092-8674(83)90201-5. [DOI] [PubMed] [Google Scholar]
  20. Sussenbach J. S., van der Vliet P. C. The mechanism of adenovirus DNA replication and the characterization of replication proteins. Curr Top Microbiol Immunol. 1984;109:53–73. doi: 10.1007/978-3-642-69460-8_2. [DOI] [PubMed] [Google Scholar]
  21. Tamanoi F., Stillman B. W. Function of adenovirus terminal protein in the initiation of DNA replication. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2221–2225. doi: 10.1073/pnas.79.7.2221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. de Vries E., van Driel W., Tromp M., van Boom J., van der Vliet P. C. Adenovirus DNA replication in vitro: site-directed mutagenesis of the nuclear factor I binding site of the Ad2 origin. Nucleic Acids Res. 1985 Jul 11;13(13):4935–4952. doi: 10.1093/nar/13.13.4935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Bergen B. G., van der Ley P. A., van Driel W., van Mansfeld A. D., van der Vliet P. C. Replication of origin containing adenovirus DNA fragments that do not carry the terminal protein. Nucleic Acids Res. 1983 Apr 11;11(7):1975–1989. doi: 10.1093/nar/11.7.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. van der Vliet P. C., van Dam D., Kwant M. M. Adenovirus DNA replication in vitro is stimulated by RNA from uninfected HeLa cells. FEBS Lett. 1984 Jun 4;171(1):5–8. doi: 10.1016/0014-5793(84)80449-4. [DOI] [PubMed] [Google Scholar]

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