FIG. 4.
Intracellular localization of 8N Gag and costaining with ER and Golgi markers. Single confocal sections are shown for cells transfected with pHXB2 expressing and 8N Gag stained with cellular markers for ER (rhodamine) and Golgi and TGN (Cy 5) (left column), or anti-p17MA antibodies (FITC; middle column). The overlays are shown on the right. (Top) 8N Gag and Grp78 (ER). (Middle) 8N Gag and p115 (cis to medial Golgi marker). Notice the distinct morphologies of the 8N Gag fluorescence (FITC; center). (Bottom) 8N Gag and TGN38. Notice that the confocal section is focused on the TGN vesicles and that 8N Gag is out of focus.