Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1980 Jun 15;188(3):749–755. doi: 10.1042/bj1880749

The action of Nupercaine on calcium efflux from rat liver mitochondria.

A P Dawson, D V Fulton
PMCID: PMC1161957  PMID: 6162452

Abstract

1. Nupercaine inhibits the Ca2+ efflux from rat liver mitochondria observed in the presence of Ruthenium Red, 50% inhibition being obtained at 80 microM-Nupercaine. 2. Neither the Ruthenium Red-stimulated efflux nor its inhibition by Nupercaine can be directly attributed to effects on mitochondrial stability. 3. Nupercaine perturbs the steady-state external Ca2+ concentration in the absence of Ruthenium Red to an extent that is explicable in terms of the inhibition of Ca2+ efflux. 4. Various factors that are likely to be involved in determining steady-state extra-mitochondrial Ca2+ concentrations are discussed.

Full text

PDF
749

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Shaikhaly M. H., Baum H. Associated effluxes of calcium and adenine nucleotides from mitochondria: mediation by throxine and other agents, and inhibition by bongkrekic acid and adenosine diphosphate [proceedings]. Biochem Soc Trans. 1979 Feb;7(1):215–216. doi: 10.1042/bst0070215. [DOI] [PubMed] [Google Scholar]
  2. Aleksandrowicz Z., Swierczyński J., Wrzolkowa T. Protective effect of nupercaine on mitochondrial structure. Biochim Biophys Acta. 1973 Apr 27;305(1):59–66. doi: 10.1016/0005-2728(73)90231-4. [DOI] [PubMed] [Google Scholar]
  3. Carafoli E. The calcium cycle of mitochondria. FEBS Lett. 1979 Aug 1;104(1):1–5. doi: 10.1016/0014-5793(79)81073-x. [DOI] [PubMed] [Google Scholar]
  4. Crompton M., Moser R., Lüdi H., Carafoli E. The interrelations between the transport of sodium and calcium in mitochondria of various mammalian tissues. Eur J Biochem. 1978 Jan 2;82(1):25–31. doi: 10.1111/j.1432-1033.1978.tb11993.x. [DOI] [PubMed] [Google Scholar]
  5. Dargel R. Effect of Ca2+ on coupling of rat liver mitochondria. FEBS Lett. 1974 May 15;42(1):57–60. doi: 10.1016/0014-5793(74)80278-4. [DOI] [PubMed] [Google Scholar]
  6. Dawson A. P., Selwyn M. J., Fulton D. V. Inhibition of Ca2+ efflux from mitochondria by nupercaine and tetracaine. Nature. 1979 Feb 8;277(5696):484–486. doi: 10.1038/277484a0. [DOI] [PubMed] [Google Scholar]
  7. Fiskum G., Lehninger A. L. Regulated release of Ca2+ from respiring mitochondria by Ca2+/2H+ antiport. J Biol Chem. 1979 Jul 25;254(14):6236–6239. [PubMed] [Google Scholar]
  8. Lehninger A. L., Vercesi A., Bababunmi E. A. Regulation of Ca2+ release from mitochondria by the oxidation-reduction state of pyridine nucleotides. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1690–1694. doi: 10.1073/pnas.75.4.1690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Low P. S., Lloyd D. H., Stein T. M., Rogers J. A., 3rd Calcium displacement by local anesthetics. Dependence on pH and anesthetic charge. J Biol Chem. 1979 May 25;254(10):4119–4125. [PubMed] [Google Scholar]
  10. Lötscher H. R., Schwerzmann K., Carafoli E. The transport of Ca2+ in a purified population of inside-out vesicles from rat liver mitochondria. FEBS Lett. 1979 Mar 1;99(1):194–198. doi: 10.1016/0014-5793(79)80277-x. [DOI] [PubMed] [Google Scholar]
  11. Mela L. Inhibition and activation of calcium transport in mitochondria. Effect of lanthanides and local anesthetic drugs. Biochemistry. 1969 Jun;8(6):2481–2486. doi: 10.1021/bi00834a034. [DOI] [PubMed] [Google Scholar]
  12. Mela L. Interactions of La3+ and local anesthetic drugs with mitochondrial Ca++ and Mn++ uptake. Arch Biochem Biophys. 1968 Feb;123(2):286–293. doi: 10.1016/0003-9861(68)90136-7. [DOI] [PubMed] [Google Scholar]
  13. Moore C. L. Specific inhibition of mitochondrial Ca++ transport by ruthenium red. Biochem Biophys Res Commun. 1971 Jan 22;42(2):298–305. doi: 10.1016/0006-291x(71)90102-1. [DOI] [PubMed] [Google Scholar]
  14. Nicholls D. G. Calcium transport and porton electrochemical potential gradient in mitochondria from guinea-pig cerebral cortex and rat heart. Biochem J. 1978 Mar 15;170(3):511–522. doi: 10.1042/bj1700511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nicholls D. G. The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by ion distribution. Eur J Biochem. 1974 Dec 16;50(1):305–315. doi: 10.1111/j.1432-1033.1974.tb03899.x. [DOI] [PubMed] [Google Scholar]
  16. Nicholls D. G. The regulation of extramitochondrial free calcium ion concentration by rat liver mitochondria. Biochem J. 1978 Nov 15;176(2):463–474. doi: 10.1042/bj1760463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pozzan T., Bragadin M., Azzone G. F. Disequilibrium between steady-state Ca2+ accumulation ratio and membrane potential in mitochondria. Pathway and role of Ca2+ efflux. Biochemistry. 1977 Dec 13;16(25):5618–5625. doi: 10.1021/bi00644a036. [DOI] [PubMed] [Google Scholar]
  18. Puskin J. S., Gunter T. E., Gunter K. K., Russell P. R. Evidence for more than one Ca2+ transport mechanism in mitochondria. Biochemistry. 1976 Aug 24;15(17):3834–3842. doi: 10.1021/bi00662a029. [DOI] [PubMed] [Google Scholar]
  19. Reed K. C., Bygrave F. L. The inhibition of mitochondrial calcium transport by lanthanides and ruthenium red. Biochem J. 1974 May;140(2):143–155. doi: 10.1042/bj1400143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rossi C., Azzi A., Azzone G. F. Ion transport in liver mitochondria. I. Metabolism-independent Ca++ binding and H+ release. J Biol Chem. 1967 Mar 10;242(5):951–957. [PubMed] [Google Scholar]
  21. Scarpa A., Azzi A. Cation binding to submitochondrial particles. Biochim Biophys Acta. 1968 Apr 29;150(3):473–481. doi: 10.1016/0005-2736(68)90147-8. [DOI] [PubMed] [Google Scholar]
  22. Scarpa A., Lindsay J. G. Maintenance of energy-linked functions in rat-liver mitochondria aged in the presence of nupercaine. Eur J Biochem. 1972 Jun 9;27(3):401–407. doi: 10.1111/j.1432-1033.1972.tb01851.x. [DOI] [PubMed] [Google Scholar]
  23. Scherphof G. L., Scarpa A., van Toorenenbergen A. The effect of local anesthetics on the hydrolysis of free and membrane-bound phospholipids catalyzed by various phospholipases. Biochim Biophys Acta. 1972 Jun 19;270(2):226–240. doi: 10.1016/0005-2760(72)90234-2. [DOI] [PubMed] [Google Scholar]
  24. Selwyn M. J., Dawson A. P., Stockdale M., Gains N. Chloride-hydroxide exchange across mitochondrial, erythrocyte and artificial lipid membranes mediated by trialkyl- and triphenyltin compounds. Eur J Biochem. 1970 May 1;14(1):120–126. doi: 10.1111/j.1432-1033.1970.tb00268.x. [DOI] [PubMed] [Google Scholar]
  25. Seppälä A. J., Saris N. E., Gauffin M. L. Inhibition of phospholipase A-induced swelling of mitochondria by local anesthetics and related agents. Biochem Pharmacol. 1971 Feb;20(2):305–313. doi: 10.1016/0006-2952(71)90065-7. [DOI] [PubMed] [Google Scholar]
  26. Stucki J. W., Ineichen E. A. Energy dissipation by calcium recycling and the efficiency of calcium transport in rat-liver mitochondria. Eur J Biochem. 1974 Oct 2;48(2):365–375. doi: 10.1111/j.1432-1033.1974.tb03778.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES