FIG. 1.
Acetylation of p53 by PCAF and p300 in vitro. GST fused to either full-length p53 (p53FL) or a p53 truncation mutant (p53ΔRD) lacking 25 amino acids from its C terminus was acetylated with either p300 HAT (left) or PCAF HAT (right). Reaction products were separated by SDS-PAGE. The gel was stained with Coomassie blue to demonstrate that equivalent substrate was used in each reaction (lower panels), and [3H]acetate reaction products were subsequently visualized by autoradiography of the identical gel (upper panels).