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Nuclear translocation of transcription factor NF-κB in human monocytes. (a and b) Monocytes were stimulated for 60 min with F and G protein individually and in combination (a) or RSV or ΔG (MOI = 1) (b), and NF-κB subunits p50 and p65 were detected in purified nuclei by a modified immunoassay. (c) Monocytes were stimulated for 60 min with RSV, ΔG, and GΔ172–187 (MOI = 1), and cytoplasmatic IκBα was detected by Western blot.
