Table 2.
Cryo-EM data collection, model refinement and validation statistics for hOGG1K249Q bound to the NCP-8-oxodGuo-137 (EMDB-19870, PDB: 9eoz)
| Data collection and processing | Dataset 1 | Dataset 2 |
|---|---|---|
| Magnification | 130000x | 130000x |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 43.76 | 39.35 |
| Defocus range (μm) | −1.1 to −2.9 | −1.1 to −2.9 |
| Pixel size (Å) | 1.045 | 1.045 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 1,134,348 | |
| Final particle images (no.) | 66,370 | |
| Map resolution (Å) | 3.1 | |
| FSC threshold | 0.143 | |
| Refinement | ||
| Initial model used PDB | 1 ebm, 3 lz0 | |
| Model composition | ||
| Non-hydrogen atoms | 14108 | |
| Protein residues | 1033 | |
| Nucleotide | 288 | |
| B factor (Å2) | ||
| Protein | 105.69 | |
| Nucleotide | 144.25 | |
| RMSDs | ||
| Bond lengths (Å) | 0.006 | |
| Bond angles (°) | 0.649 | |
| Validation | ||
| MolProbity score | 1.31 | |
| Clashscore | 4.58 | |
| Poor rotamers (%) | 0.58 | |
| Ramachandran plot | ||
| Favored (%) | 97.62 | |
| Allowed (%) | 2.38 | |
| Disallowed (%) | 0.00 | |