Fig. 2.
Loss of Kiss1R aggravates MA-induced total respiratory system resistance (Rrs). Mice from Kiss1R+/+ and Kiss1R−/− groups were exposed to increasing doses (0–50 mg/mL) of nebulized methacholine (MCh) to measure the Rrs after the PBS (A) and MA-challenge (B), using flexiVent analysis. The max Rrs at 50 mg/mL MCh dose was used to compare Kiss1R+/+ vs. Kiss1R−/− and male vs. female mice in both PBS and MA-challenged groups (C). Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. Mean ± SEM (n = 6–8 mice/group); ***p < 0.001 versus respective Kiss1R+/+ male/female PBS group; ###p < 0.001 versus respective Kiss1R−/− male/female PBS group; $$p < 0.01, $$$p < 0.001 versus respective Kiss1R+/+ male/female MA-challenged group; @@p < 0.01 versus Kiss1R−/− male MA-challenged group