TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or phenotype | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F′/endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF) U169 deoR [φ80 dlac Δ(lacZ)M15 recA1] | 49 |
| P. aeruginosa strains | ||
| PAO1 | Wild type | 19 |
| MP551 | pqsR::TnIS phoA/hah::Tc; derived from strain PAO1 | 15 |
| PAO-R1 | lasR::Tc; derived from strain PAO1 | 17 |
| PDO111 | rhlR::Tn501; derived from strain PAO1 | 2 |
| Plasmids | ||
| pACYC184 | General purpose cloning vector | 6 |
| pPCS11 | tacp-lasR on pACYC184 | 38 |
| pECP8 | tacp-lasR on pEX1.8 | This study |
| pJPP8 | tacp-rhlR on pEX1.8 | 35 |
| pDSW8 | tacp-pqsR on pEX1.8 | This study |
| pMTP58 | Minimum tiling pathway cosmid 58 which contains pqsR; Tetr | 20 |
| pLP170 | lacZ transcriptional fusion vector | 39 |
| pMWC1003 | pqsR′-lacZ transcriptional fusion | This study |
| pEX1.8 | P. aeruginosa expression vector | 35 |